Deteksi dan Prevalensi White Spot Syndrome Virus (WSSV) pada Lobster Bambu (Panulirus versicolor) Menggunakan Nested PCR di Jawa Timur
Detection and Prevalence of White Spot Syndrome Virus (WSSV) in Bamboo Lobster (Panulirus versicolor) Using Nested PCR in East Java
DOI:
https://doi.org/10.58950/jpk.v6i2.112Keywords:
deteksi molekuler, krustasea, lobster bambu, White Spot Syndrome VirusAbstract
Lobster bambu (Panulirus versicolor) merupakan komoditas krustasea bernilai ekonomi tinggi yang berpotensi menjadi pembawa berbagai agen penyakit, termasuk White Spot Syndrome Virus (WSSV). Penelitian ini bertujuan mendeteksi keberadaan WSSV dan menentukan prevalensinya pada lobster bambu yang dilalulintaskan melalui sistem karantina di Jawa Timur. Penelitian menggunakan metode deskriptif dengan pendekatan molekuler terhadap 20 ekor lobster bambu asal Sumenep, Madura, yang diperiksa pada periode November–Desember 2025. Jaringan insang dan pleopod diekstraksi menggunakan silica extraction kit dan dianalisis menggunakan nested Polymerase Chain Reaction (PCR) dengan primer 146F1/146R1 dan 146F2/146R2. Produk amplifikasi divisualisasikan melalui elektroforesis gel agarosa 1,5%. Hasil pengamatan klinis menunjukkan seluruh lobster tampak sehat tanpa gejala khas infeksi WSSV. Namun, hasil nested PCR mendeteksi satu sampel positif yang ditandai oleh pita DNA berukuran sekitar 941 bp, sedangkan 19 sampel lainnya menunjukkan hasil negatif. Berdasarkan hasil tersebut, prevalensi WSSV pada lobster bambu sebesar 5%. Temuan ini menunjukkan bahwa lobster bambu yang tampak sehat berpotensi berperan sebagai pembawa subklinis WSSV. Oleh karena itu, penerapan skrining molekuler secara rutin diperlukan untuk mendukung biosekuriti dan pengawasan lalu lintas komoditas krustasea melalui sistem karantina.
References
Ayub, F., Sarker, M., & Alam, M. S. (2007). Prevalence of White Spot Syndrome Virus Infection Detected by One-Step and Nested PCR in Selected Tiger Shrimp (Penaeus Monodon) Hatcheries. Aquaculture International, 16, 405–415. https://doi.org/10.1007/s10499-007-9153-7
Bakti, N., Dewi Shinta Achmad, Shintia N. Noho, Arif Yunitama P.I, S. St. P., & Hanifa Gobel. (2024). Identifikasi Virus WSSV (white Spot Syndrome Virus) pada Lobster Bambu (panilirus versicolor) yang Ditangkap pada Perairan Gorontalo Utara. JURNAL LEMURU, 6(3), 111–116.
Chou, H., Huang, C., Wang, C., Chiang, H., & Lo, C. (1995). Pathogenicity of a Baculovirus Infection Causing White Spot Syndrome in Cultured Penaeid Shrimp in Taiwan. Diseases of Aquatic Organisms, 23, 165–173. https://doi.org/10.3354/dao023165
Claydon, K., Cullen, B., & Owens, L. (2004). OIE White Spot Syndrome Virus PCR Gives False-Positive Results in Cherax Quadricarinatus. Diseases of Aquatic Organisms, 62, 265–268. https://doi.org/10.3354/dao062265
Durand, S. V., & Lightner, D. V. (2002). Quantitative Real Time PCR for the Measurement of White Spot Syndrome Virus in Shrimp. Journal of Fish Diseases, 25(7), 381–389. https://doi.org/10.1046/j.1365-2761.2002.00367.x
Escobedo‐Bonilla, C. M., Alday‐Sanz, V., Wille, M., Sorgeloos, P., Pensaert, M. B., & Nauwynck, H. J. (2008). A Review on the Morphology, Molecular Characterization, Morphogenesis and Pathogenesis of White Spot Syndrome Virus. Journal of Fish Diseases, 31(1), 1–18. https://doi.org/10.1111/j.1365-2761.2007.00877.x
Hidayat, N., Apada, A., Alwi, D., Amriani, R., & Rell, F. (2023). Deteksi White Spot Syndrome Virus pada Lobster Menggunakan Primer Kit IQ2000TM. Jurnal Sain Veteriner, 41, 341. https://doi.org/10.22146/jsv.76878
Islam, S. I., Mou, M. J., Sanjida, S., & Mahfuj, S. (2023). A Review on Molecular Detection Techniques of White Spot Syndrome Virus: Perspectives of Problems and Solutions in Shrimp Farming. Veterinary Medicine and Science, 9(2), 778–801. https://doi.org/10.1002/vms3.979
Kasornchandra, J., Boonyaratpalin, S., & Itami, T. (1998). Detection of White-Spot Syndrome in Cultured Penaeid Shrimp in Asia: Microscopic Observation and Polymerase Chain Reaction. Aquaculture, 164(1–4), 243–251. https://doi.org/10.1016/S0044-8486(98)00190-2
Khofifah, A., Abida, I. W., & Khusna, A. (2023). Pemeriksaan WSSV (White Syndrome Virus) dengan Uji PCR (Polymerase Chain Reaction) pada Udang Vannamei (Litopenaeus vannamei) Di UPT Laboratorium Kesehatan Ikan dan Lingkungan, Pasuruan Jawa Timur. Juvenil:Jurnal Ilmiah Kelautan Dan Perikanan, 4(2), 142–151. https://doi.org/10.21107/juvenil.v4i2.16462
Kilawati, Y., & Maimunah, Y. (2015). Kualitas Lingkungan Tambak Insentif Litapenaeus vannamei Dalam Kaitannya Dengan Prevalensi Penyakit White Spot Syndrome Virus. Research Journal of Life Science, 2(1), 50–59. https://doi.org/10.21776/ub.rjls.2015.002.01.7
Latritiani, R., Desrina, --, & Sarjito, --. (2017). Keberadaan White Spot Syndrome Virus (WSSV) pada Udang Vannamei (Litopenaeus vannamei) di Pertambakan Kota Pekalongan. In Journal of Aquaculture Management and Technology (2017th-08–28th eds., Vol. 6, Issue 3, p. 8).
Lo, C., Leu, J., Ho, C., Chen, C., Peng, S., Chen, Y., Chou, C., Yeh, P., Huang, C., Chou, H., Wang, C., & Kou, G. (1996). Detection of Baculovirus Associated with White Spot Syndrome (WSBV) in Penaeid Shrimps Using Polymerase Chain Reaction. Diseases of Aquatic Organisms, 25, 133–141. https://doi.org/10.3354/dao025133
Mahardika, K., Zafran, Z., & Koesharyani, L. (2017). Deteksi White Spot Syndrome Virus (Wssv) pada Udang Windu (Penaeus Monodon) di Bali dan Jawa Timur Menggunakan Metode Polymerase Chain Reactioin (PCR). Jurnal Penelitian Perikanan Indonesia, 10(1), 55. https://doi.org/10.15578/jppi.10.1.2004.55-59
Maryam, Izaz Ahmad, & Kainat Qadeem. (2025). Navigating Unspoken Rules: A Study of Pragmatic Failures in ESL Intercultural Communication. `, 4(01), 4220–4233.
Musthaq, S. S., Sudhakaran, R., Balasubramanian, G., & Hameed, A. S. S. (2006a). Experimental transmission and tissue tropism of White Spot Syndrome Virus (WSSV) in two species of lobsters, Panulirus homarus and Panulirus ornatus. Journal of Invertebrate Pathology, 93(2), 75–80. https://doi.org/10.1016/j.jip.2006.06.006
Musthaq, S. S., Sudhakaran, R., Balasubramanian, G., & Hameed, A. S. S. (2006b). Experimental Transmission and Tissue Tropism of White Spot Syndrome Virus (WSSV) in Two Species of Lobsters, Panulirus Homarus and Panulirus Ornatus. Journal of Invertebrate Pathology, 93(2), 75–80. https://doi.org/10.1016/j.jip.2006.06.006
Nunan, L. M., & Lightner, D. V. (2011). Optimized PCR Assay for Detection of White Spot Syndrome Virus (WSSV). Journal of Virological Methods, 171(1), 318–321. https://doi.org/10.1016/j.jviromet.2010.11.015
Pane, A. R., Reza, A., Marassabesy, I., & Suman, A. (2021). Aspek Biologi dan Status Pemanfaatan Lobster Bambu (Panulirus versicolor) di Perairan Kepulauan Aru, Maluku. BAWAL Widya Riset Perikanan Tangkap, (Vol 13, No 2 (2021): (AGUSTUS) 2021), 85–94.
Piamsomboon, P., Inchaisri, C., & Wongtavatchai, J. (2016). Climate Factors Influence the Occurrence of White Spot Disease in Cultured Penaeid Shrimp in Chanthaburi Province, Thailand. Aquaculture Environment Interactions, 8, 331–337. https://doi.org/10.3354/aei00176
Pradeep, B., Rai, P., Mohan, S. A., Shekhar, M. S., & Karunasagar, I. (2012). Biology, Host Range, Pathogenesis and Diagnosis of White spot syndrome virus. Indian Journal of Virology, 23(2), 161–174. https://doi.org/10.1007/s13337-012-0079-y
Talukder, A. S., Punom, N. J., Eshik, Md. M. E., Begum, Mst. K., Islam, H. M. R., Hossain, Z., & Rahman, M. S. (2021). Molecular Identification of White Spot Syndrome Virus (WSSV) and Associated Risk Factors for White Spot Disease (WSD) Prevalence in Shrimp (Penaeus Monodon) Aquaculture in Bangladesh. Journal of Invertebrate Pathology, 179, 107535. https://doi.org/10.1016/j.jip.2021.107535
World Organisation for Animal Health. (2023). Manual of Diagnostic Tests for Aquatic Animals: Infection with White Spot Syndrome Virus (WOAH Manual for Aquatic Animals). World Organisation for Animal Health (WOAH).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Destik Rosyana, Maria Agustin, Sumaryam
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
